David Yamaguchi ’15

Major: Biochemistry & Molecular Biology. Hometown:Erie, Colorado.


Dimensions Fellow in Research

University of Iowa Department of Biochemistry | Iowa City, Iowa

Weeks 10 and 11:
University of Iowa Department of Biochemistry


August 14, 2013 | The final weeks of my internship consisted mostly of poster work and saying goodbye to all of the wonderful members of the Baker lab.  Although I did help inject one day and helped with cloning of constructs H36 and H37, I benefitted the most from my work on the poster and review paper which both […]

Week 9:
University of Iowa Department of Biochemistry


July 23, 2013 | Things are continuing to come to a close for my experience here.  I’m still working on cloning constructs H36 and H37, and the HCN1 N terminus construct H35 is ready for injection, but the amount of experiment work I do is dwindling.  This week we injected construct H27, one of Yuan’s constructs that had low […]

Week 8:
University of Iowa Department of Biochemistry


July 16, 2013 | With Thursday and Friday included this week, I was able to accomplish a much greater amount of work.  Joe and I injected construct H28 for a second time because all of the tadpoles from the first injection lost their expression by the time we sectioned them and IHC didn’t reveal any GFP.  For the purposes […]

Week 7:
University of Iowa Department of Biochemistry


July 10, 2013 | With the Fourth of July on Thursday of this week, we only had 3 work days followed by a 4 day weekend.  This was great because the Iowa City Jazz Festival was happening downtown where I got to see numerous musicians play some great jazz.  During the work week, however, I did prepare H28, H31 […]

Week 6:
University of Iowa Department of Biochemistry


July 2, 2013 | Week six held many good things.  We finally have data. Hooray!  Yuan took some of the slides I had prepared with the tadpole sections to the confocal microscope where we took pictures of construct H30 expression.  It was so nice to see the results up close and with the greater resolution of the confocal.  What […]

Week 5:
University of Iowa Department of Biochemistry


June 24, 2013 | This week was markedly better than last week.  I got my ligation of H33 and H34 to grow colonies and found in the isolation of the plasmids that constructs H33-11 and H34-3 looked clean and ready for linearization.  These constructs were submitted for sequencing on Thursday and may be injected on Tuesday of week six […]

Week 4:
University of Iowa Department of Biochemistry


June 19, 2013 | This week was a mixed bag of good and bad as far as the science goes. We injected 3 more constructs (H29, H31, and H32) which was awesome with a reasonable and functional number of tadpoles expressing our constructs.  Our earlier construct tadpoles are now huge and will be ready for analysis in week six […]

Week 3:
University of Iowa Department of Biochemistry


June 10, 2013 | This week was referred to as “transgenic week” by the lab members due to the fact that we injected sperm nuclei (which had been modified by the reaction of the nuclei with two of our linearized plasmid constructs) into eggs this week.  And when I say eggs, I mean about five hundred for each construct.  […]

Week 2:
University of Iowa Department of Biochemistry


June 3, 2013 | This week was noticeably more rhythmic than last week.  The protocols I performed I had already done the week before and was much more comfortable than week one where absolutely everything was new.  The molecular cloning process we’re performing is done by putting different parts of the C-terminus of HCN1 onto ACTx reporter protein sequence.  […]

Week 1:
University of Iowa Department of Biochemistry


May 28, 2013 | Wow. The first week here in the Bowen Science Building (BSB) of the University of Iowa has been a great experience. The people involved, the location, and the material I’m learning about and working on have all exceeded my expectations. I had met with some of the people in the lab before the internship started […]