Chris Lopez ’13

Major: Biochemistry & Molecular Biology and Spanish. Hometown: Imperial, California.


Dimensions Fellow in Research

Carver College of Medicine, University of Iowa | Iowa City, Iowa

Week 8:
Carver College of Medicine


August 6, 2012 | On Sunday the membranes were cut into pieces and put into primary antibody and rocked overnight. Monday I washed these membranes and put them into secondary antibody. When these were done rocking I stained the AKAP1 bands with fast green. I then imaged the membranes. I also ran eight SDS-PAGE gels. Four of the gels […]

Week 7:
Carver College of Medicine


July 29, 2012 | Monday I ran four SDS-PAGE gels using the samples I made on Friday. I ran two gels for the rat brain samples and two for the liver samples. After the gels were done running I transferred them to a nitrocellulose membrane. I took the membrane and submerged it in dH2O for 5 minutes before staining […]

Week 6:
Carver College of Medicine


July 20, 2012 | On Monday I talked with Ron (who came back from vacation) about the results I had obtained. We took a look at the images of the nitrocellulose membranes for RIIalpha, RIIβ, calcineurin, and their corresponding GST-AKAP1 protein bands. The images didn’t look bad but they were a little messy. There was some background on the […]

Week 5:
Carver College of Medicine


July 13, 2012 | This week I continued making progress on my project by doing pull-downs, western blots, and protein band quantification. Tuesday I learned more about the purpose of my project. I knew that I was looking at the binding region of the PKA and AKAP1 proteins to see if the phosphorylation mutations I made would influence the […]

Week 4:
Carver College of Medicine


July 8, 2012 | As I mentioned in my past blog, Stefan (the P.I.) and Ron (the post-doc) are out of the lab on vacation. So this week I worked with Andy, the M.D./PhD. student. Over the weekend Andy grew minicultures in 5 mL conicals for the NM DNA samples. This week I will only be working with the […]

Week 3:
Carver College of Medicine


July 1, 2012 | This week went very well. I made a lot of progress on my project. On Monday I took the digests out of the freezer and added dye to all of them, including controls for the M and NM samples midiprep samples. Different master mixes with certain restriction enzymes were added to a small volume of […]

Week 2:
Carver College of Medicine


June 25, 2012 | Here are some photos from the first week. I just figured out how to decrease the file size. The first one is me about to spread E. coli cells on an ampicillin plate. The second is me about to pipette the cells onto the ampicillin plate. The third is me with Ron Merrill, the postdoc […]

Week 2:
Carver College of Medicine


June 24, 2012 | I made a lot of progress the second week of my fellowship. I learned more about the interaction between the AKAP1 and PKA proteins and more about lab techniques (digest, midipreps, minipreps, transformations, and site-directed mutagenesis). One of the best things about my second week is that I feel much more comfortable in the lab […]

Week 1:
Carver College of Medicine


June 17, 2012 | I have had a great first week to start off my fellowship. I arrived at the main office in the pharmacology department in the science building I will be working in and introduced myself to the office workers, one of which was named Linda. Linda took me to the lab where I would be working […]